TCBS Agar medium (Cholera Medium TCBS) (ISO)

Thanks to its high alkaline pH combined with citrate, bile salts, and thiosulfate , the medium effectively inhibits unwanted microorganisms, creating optimal conditions for the growth of Vibrio spp.

TCBS Agar Scharlab 500 g – Code 01-567-500

Scharlab 's TCBS Agar Selective Medium Powder 500g (product code 01-567-500 ) is a high-grade microbiological medium used for the selective isolation of pathogenic Vibrio strains , particularly Vibrio parahaemolyticus and Vibrio cholerae, according to ISO 8914 and ISO 21872-2 standards. Packaged in a 500g vacuum-sealed powder bottle , the product is suitable for research laboratories and microbiological analysis in food, water, and clinical samples .

TCBS Agar (Thiosulfate-Citrate-Bile-Sucrose Agar) offers high selectivity and differentiation , inhibiting most non- Vibrio bacteria thanks to its bile salt and citrate content, while supporting Vibrio growth due to its sucrose and characteristic alkaline pH. When used correctly, Vibrio spp. will form distinct colonies with a different color , making it easy for technicians to identify and isolate them.

Key advantages:

• ✓ High selectivity for Vibrio species in complex samples.
• ✓ Complies with international standards ISO/AOAC/FDA.
• ✓ Dry medium form, easy to prepare according to test requirements.
• ✓ Suitable for microbiological applications in food, drinking water, and medical testing.

? This product is the ideal choice for laboratories requiring an accurate and reliable Vibrio isolation environment according to international standards.

Instruct

Dissolve 88g of culture medium powder in 1 liter of distilled water. Bring to a boil and stir well. Pour into a dish. Do not autoclave.

Formula (g/L)

Proteose peptone..........................10.0

Yeast extract..................................5.0

Sodium citrate...............................10.0

Sodium thiosulphate......................10.0

Ox bile............................................8.0

Sucrose..........................................20.0

Sodium chloride.............................10.0

Ferric citrate....................................1.0

Thymol blue.....................................0.04

Bromthymol blue.............................0.04

Agar.................................................14.0